| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.045108 |
| Chromosome: | chromosome 2 |
| Location: | 6817529 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388208 | (1 of 1) 1.13.11.24 - Quercetin 2,3-dioxygenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAAACGGTGCCATGTGCCTGGTGTGAGGCCGTGACCTCACGTTCCACCT |
| Internal bar code: | TATTTCAATACGATTTTGGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2245 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGCTTCGGGTGAGTGATT |
| Suggested primer 2: | CGATAGCCGCACTCCTCATT |