| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.045137 |
| Chromosome: | chromosome 3 |
| Location: | 1665334 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g152950 | PUS12 | (1 of 1) 4.2.1.70//5.4.99.12 - Pseudouridylate synthase / Uracil hydrolyase // tRNA pseudouridine(38-40) synthase / tRNA pseudouridylate synthase I; RNA pseudouridine synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATGGACACCGGTAAGTAGTACTGGTACACGCGGCCCTCGCACATGTGG |
| Internal bar code: | GTGGTCAGTTCTTGTTAAAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4265 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 66 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAGTGAGGCCAACTTCGG |
| Suggested primer 2: | GACAACACCTCAACACACGC |