| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.045138 |
| Chromosome: | chromosome 7 |
| Location: | 2445240 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329100 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGGATTCGATTAGCTCGTCCTGGCTCGCCGACTCCCCAGACTCGCTC |
| Internal bar code: | TGGATCTTATTGCATGTGTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3132 |
| LEAP-Seq percent confirming: | 81.3953 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGTTTGCATGCCGTCAG |
| Suggested primer 2: | GCAGGATGTTGGGTTTTCGG |