| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.045161 |
| Chromosome: | chromosome 1 |
| Location: | 5471211 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038700 | CLV5 | Voltage-gated chloride channel; (1 of 2) K05016 - chloride channel 7 (CLCN7) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGCTCGGGTACGTGGCGTGCGTGTCTCATACACACATGCATGTGCAT |
| Internal bar code: | TATGTACCAATCACCGGTTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 499 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGCGGCTAGGAAGTAGAA |
| Suggested primer 2: | CATGTCACCCATTCCTGCCT |