| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.045163 |
| Chromosome: | chromosome 9 |
| Location: | 4623330 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401701 | (1 of 1) IPR001104//IPR010721 - 3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal // Protein of unknown function DUF1295 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTAAGAGCAACTAGACGAGCGCGGTTCACCAAGGAGCCGAACATAGAAT |
| Internal bar code: | TGCTCCCGCCACGTGGGTAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1595 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGTGACGAGGTGCAGGTA |
| Suggested primer 2: | TATGGGGGAGGTGAGGTGAG |