| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.045167 |
| Chromosome: | chromosome 10 |
| Location: | 2838050 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g439100 | CCT1 | T-complex protein 1, alpha subunit; (1 of 1) K09493 - T-complex protein 1 subunit alpha (CCT1, TCP1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAAAGCTCAAACTTACACGTGTCCTGAAGCGGCGTGCCTGCGGTGTGC |
| Internal bar code: | GGTCCGAGGTACCCTGGAAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2271 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGATGAGGCAGGCTCGTG |
| Suggested primer 2: | CTGGTGGACGACATTGGTGA |