| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.045176 |
| Chromosome: | chromosome 3 |
| Location: | 4044336 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171900 | FAP56 | Flagellar Associated Protein 56 | 3'UTR |
| Cre03.g171950 | PPC2,PEPC2 | (1 of 2) K01595 - phosphoenolpyruvate carboxylase (ppc); Phosphoenolpyruvate carboxylase 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATATTAATACCCTCTGCACCTTTGGCGCCCCACTGCCCCGGCGCCGCG |
| Internal bar code: | GCTAACGACGCTTGTCGTATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 412 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGGGGATGAAGACAAGAA |
| Suggested primer 2: | CCACTTCCTGTTTGTGTGCG |