Insertion junction: CLIP2.045199_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.045199
Chromosome:chromosome 1
Location:3237349
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g020800 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGTGGCGACTACGGGACCTCTTCTAACTACGGCGCGAACGCAGCCGGT
Internal bar code:AGAACGTTGTAGGATTCAGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:3463
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:100
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:100

Suggested primers for confirmation by PCR

Suggested primer 1:TACCCTGACTGCTGCTGTTG
Suggested primer 2:ATGACGATCCGCGCTAACAT