| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.045200 |
| Chromosome: | chromosome 7 |
| Location: | 1195637 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g321350 | (1 of 2) 3.1.3.1 - Alkaline phosphatase / Phosphomonoesterase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATCCGCTGAAGTCTGGGGTCAGTGATTCCCCCTTCTGACACGTTTTAT |
| Internal bar code: | AAACTCCTGGAAAGAGGGACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4242 |
| LEAP-Seq percent confirming: | 98.9011 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 91 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCTTCCTGGACTTCATGT |
| Suggested primer 2: | GTGATAGGACAAAACGGCGC |