| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.045202 |
| Chromosome: | chromosome 12 |
| Location: | 5130691 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g801426 | (1 of 726) IPR011009 - Protein kinase-like domain | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAGTCGGTTGCCGCACGCGGCACCCCTCCCTTCCTGCTCCTTCCTCCC |
| Internal bar code: | TAATGGGCCGCATATATTACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5050 |
| LEAP-Seq percent confirming: | 5.92593 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 127 |
| LEAP-Seq n unique pos: | 135 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACCTGCATGCTAGGAGAG |
| Suggested primer 2: | TGCAGCACGTGTAGGTTCAT |