| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.045211 |
| Chromosome: | chromosome 16 |
| Location: | 2171197 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g801900 | (1 of 3) K10406 - kinesin family member C2/C3 (KIFC2_3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGCAATCACCCGAGGTCCCGTCCATCGGCATGCCGGCAAGCCGTGTGC |
| Internal bar code: | TAATATTGGAGGCACGTTTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 277 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGGAGGGTTGCATTCATG |
| Suggested primer 2: | ACAAGAATTCCGACCGCCAT |