Insertion junction: CLIP2.045213_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.045213
Chromosome:chromosome 1
Location:2799378
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g017200 FKB24,FKB99 Peptidyl-prolyl cis-trans isomerase, FKBP-type; (1 of 2) PTHR10516//PTHR10516:SF290 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // PEPTIDYL-PROLYL CIS-TRANS ISOMERASE CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATTGCGGCCAACGCGGCCCGGCACCAGGCGGCGGTTCGGAAGCAAAAGA
Internal bar code:CCGTTTGCGGATATCACACCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2229
LEAP-Seq percent confirming:81.8182
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GATAGCTTCCCTCGGACACG
Suggested primer 2:TTGGCTTCGACTCATGGCAT