| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.045216 |
| Chromosome: | chromosome 2 |
| Location: | 6641587 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389901 | intron | ||
| lncRNA_TCONS_00129454 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGTAGGGTTGGGGCAGGGGTCGTCAGGGTTGGGTCGTCAGGTTTGGG |
| Internal bar code: | TTTGTTGGGGGGCAGCCATGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 152 |
| LEAP-Seq percent confirming: | 25.7143 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCACCAAGTGTAGGAGTGT |
| Suggested primer 2: | AGCACCGACACACAGCTATC |