| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.045219 |
| Chromosome: | chromosome 1 |
| Location: | 2632154 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g015700 | SMM1 | (1 of 8) 3.4.24.71 - Endothelin-converting enzyme 1 / ECE-1; S-adenosyl-L-methionine-dependent methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGACTGACGTGACGCGCTCGGGAAGAAGCCCATGCACGTCACCGCCAA |
| Internal bar code: | TTTGGAGGGTAATGTTGATGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2093 |
| LEAP-Seq percent confirming: | 72.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCCCCTAACTGCAAGCAT |
| Suggested primer 2: | TGGATGGACTTGAAGCCGTC |