Insertion junction: CLIP2.045222_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.045222
Chromosome:chromosome 11
Location:1011259
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCTGCAGTGTAAATCTGTTCGGCAGTTCGGCTGTGTGTTCGGTCCCGC
Internal bar code:CGCGGTCTTCGATTAAAAATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:2246
LEAP-Seq percent confirming:53.0201
LEAP-Seq n confirming:79
LEAP-Seq n nonconfirming:70
LEAP-Seq n unique pos:149

Suggested primers for confirmation by PCR

Suggested primer 1:CAATTCGAGGCATGCACGAG
Suggested primer 2:CAAGGAAGAAGAGGCCCCAG