Insertion junction: CLIP2.045234_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.045234
Chromosome:chromosome 2
Location:5716589
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g116400 FAP120,DII7 Flagellar Associated Protein 120; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCACGGCTTGCTCGGCCCCACATTGTACAGCTTGGATCCTGCAAACCAC
Internal bar code:CGCTTGCCTTGTGGATTTGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1956
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:GACGGGGAAGAGCAACTTGA
Suggested primer 2:CCTTCCGCTTGCGCTATCTA