| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.045253 |
| Chromosome: | chromosome 13 |
| Location: | 1494720 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572550 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGAAATGACTCTGTAAGTTGCCCGGTGGAATCCCGAAGCTAGCCGCAG |
| Internal bar code: | GACTCACGTGCTTAAAAGTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 459 |
| LEAP-Seq percent confirming: | 58.8235 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAACACGGTCGCATAATC |
| Suggested primer 2: | GACACGCACACACACTTTCC |