Insertion junction: CLIP2.045262_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.045262
Chromosome:chromosome 9
Location:6490447
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g415450 KIN17A,KIN17-1 (1 of 32) 3.6.4.4 - Plus-end-directed kinesin ATPase / Kinesin; Kinesin motor protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCTGCTGCTGTGGCGTGGTGCACTTCTCCACGATGATAACGCAAACAG
Internal bar code:CGGCCCTAGAGTTTAATTGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:214
LEAP-Seq percent confirming:28.5714
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TGCGCAGTAAGTACCTGGTG
Suggested primer 2:TTGAGTGTGGACAGCGACTC