Insertion junction: CLIP2.045268_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.045268
Chromosome:chromosome 16
Location:2447380
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g801913 (1 of 1) PTHR11700:SF11 - 40S RIBOSOMAL PROTEIN S20 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCATTGTCCTACTACTGCCAATCAGTGCCACGCCCGTATATCGGCAACAG
Internal bar code:TGACGTTGCACAGCATGAATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:900
LEAP-Seq percent confirming:21.875
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCATTCACAGCGCAGAAG
Suggested primer 2:CTCCCAGGAAGAGCAACCAG