| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.045270 |
| Chromosome: | chromosome 12 |
| Location: | 4635196 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g522150 | FAP194 | (1 of 239) IPR016024 - Armadillo-type fold; Flagellar Associated Protein 194 | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGGGGTACACCGCCGGTGGCGGGGCCGTCGGGGTCGGTAGGCTGGGG |
| Internal bar code: | TGCATCCTTGGACCGGTGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2115 |
| LEAP-Seq percent confirming: | 79.1667 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGGGCTTCGTGATGACGT |
| Suggested primer 2: | GCACCCCACCTTGATATCCC |