Insertion junction: CLIP2.045270_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.045270
Chromosome:chromosome 12
Location:4635200
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g522150 FAP194 (1 of 239) IPR016024 - Armadillo-type fold; Flagellar Associated Protein 194 CDS/intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTCGAACGCCTGGACGACCAGAATGTGATGCTGGAGGCGCTGATGAAG
Internal bar code:AGCCATAATTTAGACAGGAGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1385
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GCACCCCACCTTGATATCCC
Suggested primer 2:ATTGGGCTTCGTGATGACGT