| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.045297 |
| Chromosome: | chromosome 1 |
| Location: | 1752986 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g009700 | PPP1 | (1 of 3) PTHR12320//PTHR12320:SF15 - PROTEIN PHOSPHATASE 2C // SUBFAMILY NOT NAMED; Phosphoprotein phosphatase 2C-related | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTGAGCTCACGGCGCTGGGCGTCGCTGCTGTTGACCATGACGGCATCC |
| Internal bar code: | CACCGGCAGGCGAGGCCGCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3326 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAAGCACAGCATGACAG |
| Suggested primer 2: | TATAATACACCCCGCACGCC |