| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.045313 |
| Chromosome: | chromosome 3 |
| Location: | 6077814 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g190250 | SUB6 | (1 of 5) PTHR10795//PTHR10795:SF157 - PROPROTEIN CONVERTASE SUBTILISIN/KEXIN // MEMBRANE-BOUND TRANSCRIPTION FACTOR PEPTIDASE, SITE 1; Subtilisin-like protease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGGGCAACACCTCCTCCTGACCGGTCTCCGTGAAGGACCCATAGTCA |
| Internal bar code: | GGTCGGCTCTGCCATGATGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1761 |
| LEAP-Seq percent confirming: | 96.5517 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGACAGCACTCACACCTT |
| Suggested primer 2: | CCGGTAGAGTCAGCAAGCAA |