| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.045345 |
| Chromosome: | chromosome 17 |
| Location: | 4399275 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g730650 | (1 of 1) PTHR23250//PTHR23250:SF4 - DYSFERLIN-RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTCCCTCGGCCTGGTCCCCAGCACTGCCGCTGCCTTCAGCGCCCGCAC |
| Internal bar code: | GAGAAGTACGTGAATAATTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1318 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGTGCTGTCACCCAAGAC |
| Suggested primer 2: | TCGAGGACCAGACTCAGAGG |