Insertion junction: CLIP2.045347_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.045347
Chromosome:chromosome 9
Location:1734131
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g398400 TRP5 Transient receptor potential ion channel protein; (1 of 46) PF00520 - Ion transport protein (Ion_trans) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGCAGTGCAACAGTGACATGGTGATTGACAGCGAGTCGCTCGCCAAGG
Internal bar code:CTAACCGTCGGGTGGGGTCGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3211
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGTCGGACGTAAACCACA
Suggested primer 2:CATCCCCCTACTCCAGGTGA