Insertion junction: CLIP2.045354_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.045354
Chromosome:chromosome 16
Location:1398007
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g652150 FBP4 (1 of 1) K01103 - 6-phosphofructo-2-kinase / fructose-2,6-biphosphatase 3 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGATGGTGCGGCGCAGCGTGCTAGTCCACACCGACACGGGCACAGGCA
Internal bar code:ACGGTTAGTCCGGATTGGCGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1259
LEAP-Seq percent confirming:68.0556
LEAP-Seq n confirming:49
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:72

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTACAACCAGGTCGACCG
Suggested primer 2:GACAGTGCGTGTGTGTGTTC