Insertion junction: CLIP2.045361_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.045361
Chromosome:chromosome 10
Location:4670803
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g452000 (1 of 1) PF04752 - ChaC-like protein (ChaC) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAACTATCTGGGCCCCGCGCCACTGCCCGCGATCGCCACGCAGATCGC
Internal bar code:GTATGTGAATCGATTGCTAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1478
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GTCAGATACCCCATCGGCAG
Suggested primer 2:ATCTGGAGTGGCGGGAAAAG