| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.045399 |
| Chromosome: | chromosome 16 |
| Location: | 4613515 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g670850 | (1 of 1) PTHR22975:SF2 - ECHINUS SPLICE FORM 3 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATACTGATGCGCCGGTCCGCCTCGACACGCCCACCGCACTCACGCAC |
| Internal bar code: | TACTTTTAACGGAAAGAATAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 472 |
| LEAP-Seq percent confirming: | 6.25 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGGACGAAGAAGACGAGC |
| Suggested primer 2: | CCGTTTCCCAAGCCTACCAT |