| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.045423 |
| Chromosome: | chromosome 17 |
| Location: | 3890151 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g727700 | HEL65 | (1 of 2) K16911 - ATP-dependent RNA helicase DDX21 [EC:3.6.4.13] (DDX21); DEAD box ATP-dependent RNA helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAAGTGCTGGTGATGCTATGAGCTGGCTTCAGACTTGGTGGTAGAGTGT |
| Internal bar code: | TAATTCGTTGTATGGTGAGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 938 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCAGGTACCAGCAGAACC |
| Suggested primer 2: | CGGCCCGCTTATGATTGTTG |