| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.045427 |
| Chromosome: | chromosome 16 |
| Location: | 7977220 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g691552 | NDA1 | Type-II NADH dehydrogenase; (1 of 3) K17871 - NADH:ubiquinone reductase (non-electrogenic) (ndh1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCGGCCCACGGCTGCATGTGGTTGGAGGAGAGGGCGTGTTTTAAGGTG |
| Internal bar code: | GTAATCAGGTTTTTAGGTTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3090 |
| LEAP-Seq percent confirming: | 96.875 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAGGTACGTGGGCTTGTC |
| Suggested primer 2: | GTTCAGCACTCTGGCCAGAT |