Insertion junction: CLIP2.045446_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.045446
Chromosome:chromosome 8
Location:3833791
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g381516 (1 of 1) K11168 - dehydrogenase/reductase SDR family member 12 (DHRS12) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAATCCTACTCCCCCCCCCCACACACACATACACACGCACACACACAT
Internal bar code:ATTCCAGCGAGCTCTGTACATA

Confirmation - LEAP-Seq

LEAP-Seq distance:962
LEAP-Seq percent confirming:97.619
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:GCATGTACACATCGCAGCTG
Suggested primer 2:CGGTAGAAGGGAGAAGGGGA