Insertion junction: CLIP2.045450_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.045450
Chromosome:chromosome 17
Location:3250468
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g721750 ELG9 Exostosin-like glycosyltransferase 9; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGTCTGCCCGCCCCTTCCCAACACACACACACACACACACACACACAC
Internal bar code:TGTTAACTCCATCATTCTTGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2365
LEAP-Seq percent confirming:95.4545
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CTACTTCAGGGCCTGGTGTG
Suggested primer 2:TCCAACGAATACCCTGTGCC