Insertion junction: CLIP2.045477_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.045477
Chromosome:chromosome 1
Location:6270737
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g800113 (1 of 66) 2.7.12.1 - Dual-specificity kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTTCACCACCACCCGGGCGCCACGCCACAGGGCTGTTTACGTCGGGGAA
Internal bar code:TTGTCATGTACAACAACGGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:3718
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:CTCACCACCTCCACCAGTTC
Suggested primer 2:GAGGTGGGGGTCATGAAGTG