| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.045478 |
| Chromosome: | chromosome 2 |
| Location: | 4340849 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105200 | (1 of 2) K12382 - saposin (PSAP, SGP1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGCCGCCTCAGCTTAGCGTGACCGCGTGTCAGCATGCCGTTTCCCCCT |
| Internal bar code: | TTACTTTGTGGCCAAAGGCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1067 |
| LEAP-Seq percent confirming: | 61.2903 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGTTGCCAACAGCTTGTC |
| Suggested primer 2: | CTCGGAATCACGCCCTAGTC |