| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.045483 |
| Chromosome: | chromosome 7 |
| Location: | 675874 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g317300 | ROC78,MAPKKKK1,SIK1 | (1 of 1) PTHR24361:SF268 - SERINE/THREONINE-PROTEIN KINASE 3; Mitogen Activated Protein Kinase Kinase Kinase Kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGAACCTGGCGCGCACGCTCGCCTACAACCGCCGCTGCCTGGCGCTGCT |
| Internal bar code: | TTGGTCCCTCGATAGACCAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3249 |
| LEAP-Seq percent confirming: | 84.6154 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGGACATGTCTGCGCTTT |
| Suggested primer 2: | AGTTGGTAGGGGAAATGCCG |