Insertion junction: CLIP2.045492_4

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.045492
Chromosome:chromosome 9
Location:5371383
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g408000 POB22 (1 of 3) PF03227 - Gamma interferon inducible lysosomal thiol reductase (GILT) (GILT); Proteome of basal body 22 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCCCCGACCCAGTGCCTATGCTGCGCAACTCGGCTCGTGAGGCGCTGCG
Internal bar code:GTTTCGACTCTACGTAGGCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2151
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:100
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:100

Suggested primers for confirmation by PCR

Suggested primer 1:GCATCCTAGCTAGCTTGCGA
Suggested primer 2:CAAGACCCAAGTTGCTGCAC