| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.045499 |
| Chromosome: | chromosome 14 |
| Location: | 429772 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g610647 | (1 of 3) PF02209 - Villin headpiece domain (VHP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAATCACTTGTCTCTCCCTCACTCCTCACTGACCCTACTCTCTCTCTC |
| Internal bar code: | GTCGTACTGTATGGTTCGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 99 |
| LEAP-Seq percent confirming: | 29.1667 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTTTGGTTGCTGTTCCG |
| Suggested primer 2: | CCAGCACCATCATCACAGGT |