| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.045499 |
| Chromosome: | chromosome 14 |
| Location: | 429790 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g610647 | (1 of 3) PF02209 - Villin headpiece domain (VHP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTAAAGCAGGGATGGTGGCCGCATGATTCAGGGGGCGTCAGTGAATGA |
| Internal bar code: | TAAGTATTACTACTTAGTCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1512 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCACCATCATCACAGGT |
| Suggested primer 2: | TTGCTTTGGTTGCTGTTCCG |