| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.045513 |
| Chromosome: | chromosome 14 |
| Location: | 1539996 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617950 | CAM18 | Calmodulin-like protein; (1 of 5) PTHR23050//PTHR23050:SF90 - CALCIUM BINDING PROTEIN // CENTRIN-3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTGAACTCCACGGCATCCGGCGCGATCTTATCCATTTCCGTTTTGCCA |
| Internal bar code: | TTAGCTTTATTTGCATACCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4733 |
| LEAP-Seq percent confirming: | 87.3016 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGCAACTCGAAGTCTGCT |
| Suggested primer 2: | CCATTCCATGGTGTGAGCCT |