Insertion junction: CLIP2.045533_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.045533
Chromosome:chromosome 1
Location:2406810
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g013800 SXD1,TCY1 (1 of 1) PF14392 - Zinc knuckle (zf-CCHC_4); Zinc finger containing protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGCGGTGCAAGGGAAGATGACGTGACGACACGAGACTCATGGTCGTCTC
Internal bar code:CGCTTCATGTCGCCTAGTGCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:477
LEAP-Seq percent confirming:53.3333
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CACGCTCACCTCTCCATCTC
Suggested primer 2:CAATCCCTGGTCTGGTCCAC