Insertion junction: CLIP2.045535_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.045535
Chromosome:chromosome 7
Location:307612
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g314250 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGGGCGCGAGGTAAAAGGGGCACATAGATCGCGTGCGGTTGCGCGTGC
Internal bar code:TTGTCCTTGATCGGGTTTGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:5117
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:87
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:87

Suggested primers for confirmation by PCR

Suggested primer 1:AACCTCTCAACACCCTTGCC
Suggested primer 2:TTGTTGTTGCTGCTGCTGTG