| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.045537 |
| Chromosome: | chromosome 16 |
| Location: | 7015731 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673505 | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCGCGCCGGACCCCGTCCTACCCCGTGATTCTCCGCAATCGCATCAAA |
| Internal bar code: | TAATTCAGTGTTGTCGAGTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 262 |
| LEAP-Seq percent confirming: | 0.598802 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 166 |
| LEAP-Seq n unique pos: | 167 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGGCTGGGTTGATAACG |
| Suggested primer 2: | TACCTCGCTAGAAGCAGGGT |