| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.045540 |
| Chromosome: | chromosome 9 |
| Location: | 6060790 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412700 | (1 of 3) PF01541 - GIY-YIG catalytic domain (GIY-YIG) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGTGTAGGCCCGGTACGCTGTCACAAGGCCCGTCCAGAGCGCACCGC |
| Internal bar code: | CGTAATGGGATAATTCGTGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2263 |
| LEAP-Seq percent confirming: | 68.4211 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAAACAGCGCTGCAACCAC |
| Suggested primer 2: | GACCGCCCTGCATAGTCTAC |