Insertion junction: CLIP2.045562_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.045562
Chromosome:chromosome 13
Location:3115730
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g584700 (1 of 2) PF02636 - Putative S-adenosyl-L-methionine-dependent methyltransferase (Methyltransf_28) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCACGCCTCCTCCGCCTCCTCCTCCTCGCCGCTTGCCGCCCAGTACC
Internal bar code:CTATTCTACTCTACTAGCTTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:636
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGTTCTTCTTCGGCGACT
Suggested primer 2:CGACTACGAGCGAGACTCAC