Insertion junction: CLIP2.045563_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.045563
Chromosome:chromosome 12
Location:4237996
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g518107 (1 of 1) PF04182 - B-block binding subunit of TFIIIC (B-block_TFIIIC) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCACCTCTCCCGCGCCCACACCCGCCCTCACTATCCCACCCTCACTCT
Internal bar code:GGATACTGGGGAGGAAGGAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:105
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AGGAAACCGACACGACACTC
Suggested primer 2:ATGAGTCGTCTGCTGCTGTC