Insertion junction: CLIP2.045565_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.045565
Chromosome:chromosome 13
Location:2622192
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g581000 GT90F26,GT90-26 GT90 family protein 26; (1 of 11) 2.4.2.26 - Protein xylosyltransferase / Uridine diphosphoxylose-protein xylosyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTTGCGCCCACTCGTGCTCTGCGGGCGCGAGGTGACCGACTGGAGTCT
Internal bar code:GGTTTCGTCTCACAGGCCAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1770
LEAP-Seq percent confirming:96.0
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:TTACGCGACGTCACACAGAA
Suggested primer 2:CACCAGTTTGAACACTGCCG