| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.045577 |
| Chromosome: | chromosome 10 |
| Location: | 4569611 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451500 | SRR28,SRR28A,CHI5 | Scavenger receptor cysteine rich (SRCR) protein; (1 of 20) IPR001190//IPR017448 - SRCR domain // SRCR-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGTGAGTGGCATGTTTGGCGATGCGTACGGACACACGTGCGGGCAAGT |
| Internal bar code: | AAGCGCATGGTCTTTTTCAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3929 |
| LEAP-Seq percent confirming: | 97.7011 |
| LEAP-Seq n confirming: | 85 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 87 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCTTGTTGTTCCGGTTG |
| Suggested primer 2: | GACTCAGCTGGGAAGCAGTT |