Insertion junction: CLIP2.045581_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.045581
Chromosome:chromosome 6
Location:2954001
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g273450 POB11 Proteome of basal body 11 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACCTCTCGTGCGCGATTGGGTCCGCACCATCCTGCGCCACGAACCCAA
Internal bar code:ATCACCTGCTTTGGTCCCGACC

Confirmation - LEAP-Seq

LEAP-Seq distance:4286
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:99
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:99

Suggested primers for confirmation by PCR

Suggested primer 1:CTACTAGACACGAGCTGGCG
Suggested primer 2:GTGAAAGCGTAGTGGGGGAA