| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.045593 |
| Chromosome: | chromosome 3 |
| Location: | 8236636 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200000 | (1 of 2) PTHR10183//PTHR10183:SF323 - CALPAIN // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACTATAGTAGCACTGTAATATCTTGTTTGCTGGAGCTGTAGGGGCCA |
| Internal bar code: | TAGAGGGGCCATATGTTGATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1404 |
| LEAP-Seq percent confirming: | 96.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCTGCGAAAGAAACCGTA |
| Suggested primer 2: | TGGCCTCCAAGTGTTCGTAC |