| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.045598 |
| Chromosome: | chromosome 10 |
| Location: | 5955615 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461250 | NSF1 | (1 of 1) K06027 - vesicle-fusing ATPase (NSF) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCAAGCGAGGTAGGAGACAGGTGCGTGTAGAATGGGAGGAGGTGGATA |
| Internal bar code: | TAAGGTATCCTCGCGGGTAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1565 |
| LEAP-Seq percent confirming: | 96.875 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGTTCGCGTCGATCGAC |
| Suggested primer 2: | CGGTAGCCCCTTTGCCTTAA |